BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
ARHGDIA
Bles01
Rho GDP dissociation inhibitor (GDI) alpha
GO Process (9)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
- Rho protein signal transduction [ISO]
- cellular response to mechanical stimulus [IMP]
- cellular response to organic cyclic compound [IEP]
- cellular response to redox state [IEP]
- negative regulation of cell migration [IMP]
- regulation of catalytic activity [ISO]
- regulation of cell shape [IMP]
- regulation of protein localization [ISO]
- semaphorin-plexin signaling pathway [ISO, ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID