BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
DLG1
Dlgh1, SAP97
discs, large homolog 1 (Drosophila)
GO Process (34)
GO Function (11)
GO Component (30)
Gene Ontology Biological Process
- T cell activation [ISO]
- T cell cytokine production [ISO]
- actin filament organization [ISO, ISS]
- activation of protein kinase activity [ISO]
- amyloid precursor protein metabolic process [ISO]
- branching involved in ureteric bud morphogenesis [ISO]
- cell adhesion [IEP]
- cortical actin cytoskeleton organization [ISO, ISS]
- embryonic skeletal system morphogenesis [ISO]
- endothelial cell proliferation [ISO, ISS]
- hard palate development [ISO]
- immunological synapse formation [ISO]
- lens development in camera-type eye [ISO]
- membrane raft organization [ISO]
- negative regulation of T cell proliferation [ISO]
- negative regulation of epithelial cell proliferation [ISO]
- negative regulation of mitotic cell cycle [ISO, ISS]
- negative regulation of protein kinase B signaling [ISO]
- peristalsis [ISO]
- positive regulation of actin filament polymerization [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of establishment of protein localization to plasma membrane [ISO]
- positive regulation of potassium ion transport [IMP, ISO]
- protein localization [ISO]
- protein localization to plasma membrane [ISO]
- regulation of membrane potential [ISO]
- regulation of myelination [ISO]
- regulation of protein localization [IMP]
- reproductive structure development [ISO]
- single organismal cell-cell adhesion [ISO, ISS]
- smooth muscle tissue development [ISO]
- tight junction assembly [ISO]
- tissue morphogenesis [ISO]
- ureteric bud development [ISO]
Gene Ontology Molecular Function- L27 domain binding [ISO]
- PDZ domain binding [IPI]
- ion channel binding [ISO]
- ionotropic glutamate receptor binding [IPI]
- mitogen-activated protein kinase kinase binding [ISO]
- phosphatase binding [ISO, ISS]
- potassium channel regulator activity [ISO]
- protein C-terminus binding [IPI, ISO]
- protein binding [IPI]
- protein complex scaffold [ISO]
- protein kinase binding [IPI, ISO, ISS]
- L27 domain binding [ISO]
- PDZ domain binding [IPI]
- ion channel binding [ISO]
- ionotropic glutamate receptor binding [IPI]
- mitogen-activated protein kinase kinase binding [ISO]
- phosphatase binding [ISO, ISS]
- potassium channel regulator activity [ISO]
- protein C-terminus binding [IPI, ISO]
- protein binding [IPI]
- protein complex scaffold [ISO]
- protein kinase binding [IPI, ISO, ISS]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- MPP7-DLG1-LIN7 complex [ISO]
- T-tubule [IDA]
- basal lamina [ISO]
- basal plasma membrane [IDA]
- basolateral plasma membrane [IDA, ISO, ISS]
- cell junction [ISO]
- cell projection membrane [ISO]
- cell-cell junction [ISO]
- cytoplasm [ISO]
- cytoplasmic side of plasma membrane [ISO]
- endoplasmic reticulum [ISO]
- extracellular vesicular exosome [ISO]
- immunological synapse [ISO]
- lateral loop [ISO]
- lateral plasma membrane [ISO]
- membrane raft [ISO]
- microtubule [ISO]
- myelin sheath abaxonal region [ISO]
- neuromuscular junction [ISO]
- neuron projection [IDA]
- node of Ranvier [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [IDA, ISO, TAS]
- postsynaptic membrane [TAS]
- presynaptic membrane [IDA]
- synapse [ISO]
- tight junction [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID