BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
PPP3CA
Calna1
protein phosphatase 3, catalytic subunit, alpha isozyme
GO Process (21)
GO Function (10)
GO Component (9)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [ISO]
- calcineurin-NFAT signaling cascade [ISO]
- calcium ion transport [ISO]
- calcium-mediated signaling [ISO]
- cellular response to drug [ISO]
- cellular response to glucose stimulus [IMP]
- dephosphorylation [ISO]
- multicellular organismal response to stress [ISO]
- negative regulation of calcium ion-dependent exocytosis [IC]
- negative regulation of insulin secretion [IMP]
- positive regulation of NFAT protein import into nucleus [ISO]
- positive regulation of cardiac muscle hypertrophy in response to stress [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- protein dephosphorylation [IDA, ISO]
- protein import into nucleus [ISO]
- regulation of excitatory postsynaptic membrane potential [ISO]
- regulation of synaptic transmission [ISO]
- response to amphetamine [IEP]
- response to calcium ion [ISO, ISS]
- skeletal muscle fiber development [ISO]
- transition between fast and slow fiber [ISO]
Gene Ontology Molecular Function- calcium-dependent protein serine/threonine phosphatase activity [IDA]
- calmodulin binding [IDA, ISO]
- calmodulin-dependent protein phosphatase activity [ISO, ISS]
- drug binding [ISO]
- enzyme binding [ISO]
- phosphoprotein phosphatase activity [IDA, ISO]
- protein binding [IPI]
- protein dimerization activity [ISO]
- protein heterodimerization activity [IDA]
- protein serine/threonine phosphatase activity [IDA, TAS]
- calcium-dependent protein serine/threonine phosphatase activity [IDA]
- calmodulin binding [IDA, ISO]
- calmodulin-dependent protein phosphatase activity [ISO, ISS]
- drug binding [ISO]
- enzyme binding [ISO]
- phosphoprotein phosphatase activity [IDA, ISO]
- protein binding [IPI]
- protein dimerization activity [ISO]
- protein heterodimerization activity [IDA]
- protein serine/threonine phosphatase activity [IDA, TAS]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID