BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
PPP1CA
protein phosphatase 1, catalytic subunit, alpha isozyme
GO Process (9)
GO Function (5)
GO Component (14)
Gene Ontology Biological Process
- branching morphogenesis of an epithelial tube [ISO]
- circadian regulation of gene expression [ISO, ISS]
- entrainment of circadian clock by photoperiod [ISO, ISS]
- lung development [ISO]
- positive regulation of extrinsic apoptotic signaling pathway in absence of ligand [ISO]
- protein dephosphorylation [IDA, ISO]
- regulation of circadian rhythm [ISO, ISS]
- regulation of glycogen biosynthetic process [IDA]
- regulation of glycogen catabolic process [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- MLL5-L complex [ISO]
- PTW/PP1 phosphatase complex [ISO, ISS]
- cytoplasm [IDA, ISO]
- cytosol [TAS]
- dendritic spine [IDA]
- extracellular vesicular exosome [ISO]
- glycogen granule [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- perikaryon [IDA]
- plasma membrane [ISO]
- protein phosphatase type 1 complex [IDA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID