BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
SNAP25
SNAP-25B, SNAP-25a
synaptosomal-associated protein 25
GO Process (17)
GO Function (9)
GO Component (28)
Gene Ontology Biological Process
- axonogenesis [IEP]
- calcium ion-dependent exocytosis of neurotransmitter [IDA]
- endosomal transport [IDA]
- exocytosis [TAS]
- growth hormone secretion [IEP]
- long-term memory [IMP]
- long-term synaptic potentiation [ISO]
- neurotransmitter secretion [ISO]
- positive regulation of hormone secretion [IMP]
- positive regulation of insulin secretion [IEP]
- potassium ion transmembrane transport [IDA]
- regulation of establishment of protein localization [ISO]
- regulation of neuron projection development [IMP]
- regulation of synapse assembly [IEP]
- sleep [IDA]
- synaptic vesicle fusion to presynaptic membrane [IBA]
- synaptic vesicle priming [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- BLOC-1 complex [ISO, ISS]
- SNARE complex [IDA, ISO]
- actin cytoskeleton [IDA]
- axon [IDA]
- axonal growth cone [IDA]
- cell [ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA]
- endosome [IDA]
- filopodium [IDA]
- growth cone [IDA]
- intracellular organelle [IDA]
- lamellipodium [IDA]
- membrane [ISO, ISS]
- membrane raft [IDA]
- myelin sheath [ISO]
- neuron projection [IDA]
- neuronal cell body [IDA]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA]
- synapse [ISO]
- synaptobrevin 2-SNAP-25-syntaxin-1a complex [IDA]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex [IDA, ISO]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex [IDA]
- terminal bouton [IDA]
- trans-Golgi network [ISO]
- vesicle [ISO]
- voltage-gated potassium channel complex [IDA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID