BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
ATP1A1
Nkaa1b
ATPase, Na+/K+ transporting, alpha 1 polypeptide
GO Process (27)
GO Function (13)
GO Component (19)
Gene Ontology Biological Process
- ATP catabolic process [ISO, TAS]
- cardiac muscle contraction [TAS]
- cellular potassium ion homeostasis [ISO]
- cellular response to mechanical stimulus [IDA]
- cellular response to steroid hormone stimulus [ISO]
- cellular sodium ion homeostasis [ISO]
- dephosphorylation [ISO]
- energy coupled proton transmembrane transport, against electrochemical gradient [TAS]
- membrane hyperpolarization [IMP]
- membrane repolarization [ISO]
- negative regulation of glucocorticoid biosynthetic process [ISO]
- negative regulation of heart contraction [ISO]
- positive regulation of heart contraction [ISO]
- positive regulation of striated muscle contraction [ISO]
- potassium ion import [IDA, ISO]
- potassium ion transmembrane transport [ISO]
- potassium ion transport [IDA]
- regulation of blood pressure [ISO]
- regulation of cardiac muscle cell contraction [IMP]
- regulation of sodium ion transport [IDA]
- regulation of the force of heart contraction [ISO]
- relaxation of cardiac muscle [TAS]
- response to drug [ISO]
- response to glycoside [ISO]
- sodium ion export from cell [ISO]
- sodium ion transmembrane transport [IDA, ISO]
- sodium ion transport [IDA]
Gene Ontology Molecular Function- ADP binding [IDA]
- ATP binding [IDA]
- ankyrin binding [IPI]
- chaperone binding [IDA, ISO]
- phosphatase activity [ISO]
- phosphatidylinositol 3-kinase binding [IPI]
- potassium ion binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- sodium ion binding [IDA]
- sodium:potassium-exchanging ATPase activity [IDA, ISO]
- steroid hormone binding [ISO]
- ADP binding [IDA]
- ATP binding [IDA]
- ankyrin binding [IPI]
- chaperone binding [IDA, ISO]
- phosphatase activity [ISO]
- phosphatidylinositol 3-kinase binding [IPI]
- potassium ion binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- sodium ion binding [IDA]
- sodium:potassium-exchanging ATPase activity [IDA, ISO]
- steroid hormone binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- T-tubule [IDA, ISO]
- apical plasma membrane [ISO]
- basolateral plasma membrane [ISO]
- caveola [IDA]
- endoplasmic reticulum [IDA]
- endosome [IDA]
- extracellular vesicular exosome [ISO]
- integral component of membrane [ISS]
- intercalated disc [IDA]
- membrane [IDA, ISO]
- membrane raft [IDA]
- myelin sheath [ISO]
- neuronal postsynaptic density [ISO]
- plasma membrane [IDA, ISO]
- protein complex [ISO]
- sarcolemma [IDA, ISO]
- sodium:potassium-exchanging ATPase complex [IDA, ISO]
- vesicle [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID