BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
SYT1
P65
synaptotagmin I
GO Process (13)
GO Function (15)
GO Component (13)
Gene Ontology Biological Process
- calcium ion-dependent exocytosis [TAS]
- detection of calcium ion [IC, IDA]
- fast, calcium ion-dependent exocytosis of neurotransmitter [ISS]
- neurotransmitter secretion [ISO]
- positive regulation of calcium ion-dependent exocytosis [IDA]
- positive regulation of synaptic transmission [ISS]
- positive regulation of vesicle fusion [IDA]
- regulation of calcium ion-dependent exocytosis [IMP]
- regulation of regulated secretory pathway [IMP]
- regulation of synaptic transmission, glutamatergic [ISS]
- response to calcium ion [IDA]
- synaptic vesicle exocytosis [ISO, TAS]
- vesicle docking [IDA]
Gene Ontology Molecular Function- SNARE binding [IDA, IPI]
- calcium ion binding [IDA, IMP, ISO, TAS]
- calcium-dependent phospholipid binding [IDA]
- calcium-dependent protein binding [IDA, ISO]
- calmodulin binding [IDA]
- clathrin binding [IDA]
- identical protein binding [IPI]
- low-density lipoprotein particle receptor binding [ISO]
- phosphatidylinositol-4,5-bisphosphate binding [IDA]
- phosphatidylserine binding [IDA]
- phospholipid binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- syntaxin binding [IDA]
- syntaxin-1 binding [IDA]
- SNARE binding [IDA, IPI]
- calcium ion binding [IDA, IMP, ISO, TAS]
- calcium-dependent phospholipid binding [IDA]
- calcium-dependent protein binding [IDA, ISO]
- calmodulin binding [IDA]
- clathrin binding [IDA]
- identical protein binding [IPI]
- low-density lipoprotein particle receptor binding [ISO]
- phosphatidylinositol-4,5-bisphosphate binding [IDA]
- phosphatidylserine binding [IDA]
- phospholipid binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- syntaxin binding [IDA]
- syntaxin-1 binding [IDA]
Gene Ontology Cellular Component
- SNARE complex [IDA]
- cell [ISO]
- cytoplasm [IDA, TAS]
- dense core granule [IDA]
- excitatory synapse [IDA]
- intracellular organelle [IDA]
- neuron projection [ISO]
- plasma membrane [IDA]
- presynaptic membrane [ISO]
- secretory granule [IDA, ISO]
- synaptic vesicle [IDA, ISO]
- synaptic vesicle membrane [ISO, TAS]
- terminal bouton [IDA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID