BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
OGT
O-linked N-acetylglucosamine (GlcNAc) transferase
GO Process (41)
GO Function (12)
GO Component (13)
Gene Ontology Biological Process
- apoptotic process [ISO, ISS]
- cellular response to glucose stimulus [IMP]
- cellular response to insulin stimulus [IEP]
- cellular response to lipopolysaccharide [IMP]
- cellular response to retinoic acid [ISO]
- cellular response to toxic substance [IEP]
- circadian regulation of gene expression [ISO, ISS]
- forebrain development [IEP]
- glucosamine metabolic process [IDA, IMP]
- histone H3-K4 trimethylation [ISO, ISS]
- histone H4-K16 acetylation [ISO, ISS]
- histone H4-K5 acetylation [ISO, ISS]
- histone H4-K8 acetylation [ISO, ISS]
- intracellular distribution of mitochondria [IMP]
- negative regulation of cell death [IMP]
- negative regulation of cellular response to hypoxia [IMP]
- negative regulation of peptidyl-serine phosphorylation [IMP]
- negative regulation of peptidyl-threonine phosphorylation [IMP]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of protein targeting to membrane [IMP]
- negative regulation of protein ubiquitination [ISO, ISS]
- phosphatidylinositol-mediated signaling [ISO, ISS]
- positive regulation of catalytic activity [ISO]
- positive regulation of cell size [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of granulocyte differentiation [ISO]
- positive regulation of histone H3-K27 methylation [ISO, ISS]
- positive regulation of histone H3-K4 methylation [ISO]
- positive regulation of protein localization to nucleus [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of proteolysis [ISO, ISS]
- positive regulation of reactive oxygen species biosynthetic process [IMP]
- positive regulation of transcription from RNA polymerase II promoter [ISO, ISS]
- protein O-linked glycosylation [IDA, IMP, ISO]
- protein heterotrimerization [IDA]
- protein homotrimerization [IDA]
- regulation of Rac protein signal transduction [ISO]
- regulation of gluconeogenesis involved in cellular glucose homeostasis [ISO, ISS]
- regulation of glycolytic process [ISO, ISS]
- regulation of insulin receptor signaling pathway [ISO]
- response to insulin [ISO]
Gene Ontology Molecular Function- enzyme activator activity [ISO]
- histone acetyltransferase activity (H4-K16 specific) [ISO, ISS]
- histone acetyltransferase activity (H4-K5 specific) [ISO, ISS]
- histone acetyltransferase activity (H4-K8 specific) [ISO, ISS]
- monosaccharide binding [IDA]
- peptide binding [IDA]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO, ISS]
- protein N-acetylglucosaminyltransferase activity [IDA, ISO]
- protein O-GlcNAc transferase activity [ISO, ISS]
- protein binding [IPI]
- protein domain specific binding [IPI]
- transcription factor binding [IPI]
- enzyme activator activity [ISO]
- histone acetyltransferase activity (H4-K16 specific) [ISO, ISS]
- histone acetyltransferase activity (H4-K5 specific) [ISO, ISS]
- histone acetyltransferase activity (H4-K8 specific) [ISO, ISS]
- monosaccharide binding [IDA]
- peptide binding [IDA]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO, ISS]
- protein N-acetylglucosaminyltransferase activity [IDA, ISO]
- protein O-GlcNAc transferase activity [ISO, ISS]
- protein binding [IPI]
- protein domain specific binding [IPI]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
- MLL5-L complex [ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- euchromatin [IDA]
- histone acetyltransferase complex [ISO, ISS]
- microtubule organizing center [ISO]
- mitochondrion [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO, ISS]
- zymogen granule [IDA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID