BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
ANK3
ANK-3
ankyrin 3, node of Ranvier (ankyrin G)
GO Process (23)
GO Function (7)
GO Component (25)
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [ISO]
- axon guidance [ISO]
- axonogenesis [ISO]
- establishment of protein localization [ISO]
- maintenance of protein location in plasma membrane [ISO]
- membrane assembly [IGI, ISO]
- mitotic cytokinesis [ISO]
- neuromuscular junction development [IEP]
- neuronal action potential [ISO]
- plasma membrane organization [ISO]
- positive regulation of cation channel activity [IMP]
- positive regulation of cell communication by electrical coupling [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of homotypic cell-cell adhesion [IMP]
- positive regulation of membrane depolarization during cardiac muscle cell action potential [IMP]
- positive regulation of membrane potential [IMP]
- positive regulation of protein targeting to membrane [IMP]
- positive regulation of sodium ion transmembrane transporter activity [IMP]
- positive regulation of sodium ion transport [IMP]
- protein localization to plasma membrane [IGI, IMP, ISO]
- protein targeting to plasma membrane [ISO]
- regulation of potassium ion transport [IDA]
- synapse organization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- T-tubule [IDA]
- Z disc [IDA]
- axon [ISO]
- axon initial segment [IDA, ISO]
- basal plasma membrane [ISO]
- basolateral plasma membrane [ISO]
- cell [ISO]
- cell surface [IDA]
- costamere [TAS]
- dendrite [IDA]
- intercalated disc [IDA, ISO]
- intracellular [ISO]
- lateral plasma membrane [ISO]
- membrane [ISO]
- neuromuscular junction [IDA]
- neuron projection [IDA]
- node of Ranvier [IDA, ISO]
- paranode region of axon [ISO]
- plasma membrane [IDA]
- postsynaptic membrane [IDA]
- sarcolemma [IDA, ISO]
- sarcoplasmic reticulum [IDA]
- spectrin-associated cytoskeleton [IDA]
- synapse [ISO]
- tight junction [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID