BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
VAMP3
vesicle-associated membrane protein 3
GO Process (15)
GO Function (4)
GO Component (15)
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [ISO]
- SNARE complex assembly [ISO]
- calcium ion-dependent exocytosis [ISO]
- exocytosis [IBA]
- mucus secretion [ISO]
- negative regulation of secretion by cell [ISO]
- neurotransmitter secretion [IBA]
- positive regulation of immunoglobulin secretion [ISO]
- positive regulation of receptor recycling [ISO, ISS]
- protein complex assembly [IDA]
- regulation of histamine secretion by mast cell [ISO]
- retrograde transport, endosome to Golgi [ISO, ISS]
- substrate adhesion-dependent cell spreading [ISO, ISS]
- vesicle fusion [IBA]
- vesicle-mediated transport [ISO, ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IDA, ISO]
- apical plasma membrane [IDA]
- cell [ISO]
- cell surface [ISO]
- clathrin-coated vesicle [ISO]
- clathrin-coated vesicle membrane [IDA]
- cytoplasmic vesicle [ISO]
- cytosol [ISO]
- intracellular [ISO]
- intracellular membrane-bounded organelle [IDA, ISO]
- intracellular organelle [IDA]
- plasma membrane [IDA, ISO, ISS]
- recycling endosome [IDA, ISO]
- secretory granule [ISO]
- synaptic vesicle [IBA]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID