BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
VAMP7
Sybl1
vesicle-associated membrane protein 7
GO Process (19)
GO Function (3)
GO Component (24)
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [IDA]
- Golgi to plasma membrane protein transport [ISO]
- SNARE complex assembly [ISO]
- autophagic vacuole fusion [ISO]
- calcium ion-dependent exocytosis [IDA]
- endosome to lysosome transport [IDA, ISO]
- eosinophil degranulation [IDA, ISO]
- exocytosis [IDA]
- natural killer cell degranulation [ISO]
- neutrophil degranulation [IDA, ISO]
- phagocytosis, engulfment [IDA]
- positive regulation of dendrite morphogenesis [ISO]
- positive regulation of histamine secretion by mast cell [ISO]
- regulation of protein targeting to vacuolar membrane [ISO]
- triglyceride transport [IMP]
- vesicle fusion [IDA, ISO]
- vesicle fusion with Golgi apparatus [IDA]
- vesicle transport along microtubule [IDA]
- vesicle-mediated transport [IDA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- SNARE complex [IDA, ISO]
- apical part of cell [ISO]
- azurophil granule membrane [ISO]
- cell [ISO]
- cell surface [ISO]
- cytoplasm [ISO]
- endoplasmic reticulum membrane [IDA]
- extracellular vesicular exosome [ISO]
- intracellular [ISO]
- intracellular membrane-bounded organelle [ISO]
- lamellipodium [ISO]
- late endosome membrane [IDA]
- lysosomal membrane [IDA, ISO]
- membrane [ISO]
- neuron projection [IDA, ISO, ISS]
- perinuclear region of cytoplasm [ISO]
- phagocytic vesicle [ISO, ISS]
- plasma membrane [ISO]
- platelet alpha granule [ISO]
- pseudopodium [ISO]
- secretory granule [ISO]
- secretory granule membrane [ISO]
- trans-Golgi network [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID