BAIT
UBC
ubiquitin C
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
VCL
vinculin
GO Process (10)
GO Function (4)
GO Component (16)
Gene Ontology Biological Process
- adherens junction assembly [ISO]
- apical junction assembly [ISO]
- axon extension [ISO]
- cell adhesion [ISO]
- epithelial cell-cell adhesion [ISO]
- lamellipodium assembly [ISO]
- morphogenesis of an epithelium [ISO]
- platelet aggregation [ISO]
- protein localization to cell surface [ISO]
- regulation of cell migration [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Z disc [IDA]
- actin filament [IDA]
- adherens junction [ISO]
- cell-cell adherens junction [ISO]
- cell-cell junction [ISO]
- costamere [ISO]
- cytoplasm [ISO]
- extracellular vesicular exosome [ISO]
- fascia adherens [ISO]
- focal adhesion [IDA, ISO]
- intercalated disc [IDA]
- plasma membrane [ISO]
- protein complex [ISO]
- sarcolemma [IDA]
- stress fiber [IDA]
- vesicle [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Synaptic Protein Ubiquitination in Rat Brain Revealed by Antibody-based Ubiquitome Analysis.
Protein ubiquitination is an essential post-translational modification regulating neurodevelopment, synaptic plasticity, learning, and memory, and its dysregulation contributes to the pathogenesis of neurological diseases. Here we report a systematic analysis of ubiquitinated proteome (ubiquitome) in rat brain using a newly developed monoclonal antibody that recognizes the diglycine tag on lysine residues in trypsinized peptides (K-GG peptides). Initial antibody specificity analysis ... [more]
J. Proteome Res. Aug. 15, 2012; 0(0); [Pubmed: 22871113]
Throughput
- Low Throughput
Curated By
- BioGRID