BAIT

APOA1

apolipoprotein A-I
GO Process (44)
GO Function (12)
GO Component (16)

Gene Ontology Biological Process

Homo sapiens

Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Distribution and localization of lecithin:cholesterol acyltransferase and cholesteryl ester transfer activity in A-I-containing lipoproteins.

Cheung MC, Wolf AC, Lum KD, Tollefson JH, Albers JJ

Two types of A-I-containing lipoproteins are found in human high density lipoproteins (HDL): particles with A-II (Lp(A-I with A-II] and particles without A-II (Lp(A-I without A-II]. We have studied the distribution of lecithin:cholesterol acyltransferase (LCAT) and cholesteryl ester transfer (CET) activities in these particles. Lp(A-I with A-II) and Lp(A-I without A-II) particles were isolated from ten normolipidemic subjects by anti-A-I ... [more]

J. Lipid Res. Nov. 01, 1986; 27(11);1135-44 [Pubmed: 3104518]

Throughput

  • Low Throughput

Ontology Terms

  • blood (UBERON:0000178)
  • blood (BTO:0000089)

Additional Notes

  • affinity purification of APOA1 particles with (or without) APOA2 also brings down APO D lipoprotein

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
APOD APOA1
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
-

Curated By

  • BioGRID