An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Localized domains of G9a-mediated histone methylation are required for silencing of neuronal genes.

Roopra A, Qazi R, Schoenike B, Daley TJ, Morrison JF

Negative regulation of transcription is an important strategy in establishing and maintaining cell-specific gene expression patterns. Many neuronal genes are subject to active transcriptional repression outside the nervous system to establish neuronal specificity. NRSF/REST has been demonstrated to regulate at least 30 genes and contribute to their neuronal targeting by repressing transcription outside the nervous system. Further, human genome database ... [more]

Mol. Cell Jun. 18, 2004; 14(6);727-38 [Pubmed: 15200951]

Throughput

Low Throughput

Additional Notes

figure 6.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CBX1 EHMT2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3350816
CBX1 EHMT2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hauri S (2016)	High	-	BioGRID	-
EHMT2 CBX1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Marcon E (2014)	High	23.1915	BioGRID	2945503
EHMT2 CBX1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Chin HG (2007)	Low	-	BioGRID	-
EHMT2 CBX1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Spektor TM (2009)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

EHMT2

Gene Ontology Biological Process

Gene Ontology Molecular Function

C2H2 zinc finger domain binding [IPI]histone methyltransferase activity (H3-K27 specific) [ISS]histone methyltransferase activity (H3-K9 specific) [ISS]histone-lysine N-methyltransferase activity [IMP]p53 binding [IPI]protein binding [IPI]protein-lysine N-methyltransferase activity [IDA]

Gene Ontology Cellular Component

CBX1

Gene Ontology Molecular Function

chromatin binding [TAS]enzyme binding [IPI]histone methyltransferase binding [IPI]protein binding [IPI]