BAIT

BBS4

AW537059, AW742241, D9Ertd464e
Bardet-Biedl syndrome 4 (human)
GO Process (34)
GO Function (6)
GO Component (12)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Essential role for the Prader-Willi syndrome protein necdin in axonal outgrowth.

Lee S, Walker CL, Karten B, Kuny SL, Tennese AA, O'Neill MA, Wevrick R

Necdin and Magel2 are related proteins inactivated in Prader-Willi syndrome (PWS), a sporadic chromosomal deletion disorder. We demonstrate that necdin and Magel2 bind to and prevent proteasomal degradation of Fez1, a fasciculation and elongation protein implicated in axonal outgrowth and kinesin-mediated transport, and also bind to the Bardet-Biedl syndrome (BBS) protein BBS4 in co-transfected cells. The interactions among necdin, Magel2, ... [more]

Hum. Mol. Genet. Mar. 01, 2005; 14(5);627-37 [Pubmed: 15649943]

Throughput

  • Low Throughput

Curated By

  • BioGRID