HUNK
Gene Ontology Biological Process
Gene Ontology Molecular Function
NEDD4
Gene Ontology Biological Process
- T cell activation [ISO]
- adaptive immune response [ISO]
- blood vessel morphogenesis [ISO]
- cellular response to UV [ISO]
- development involved in symbiotic interaction [ISO]
- endocardial cushion development [ISO]
- glucocorticoid receptor signaling pathway [ISO]
- immune response [IEP]
- lysosomal transport [ISO]
- negative regulation of sodium ion transport [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage [ISO]
- negative regulation of vascular endothelial growth factor receptor signaling pathway [ISO, ISS]
- neuromuscular junction development [ISO]
- neuron projection development [ISO]
- outflow tract morphogenesis [ISO]
- positive regulation of nucleocytoplasmic transport [ISO]
- positive regulation of phosphatidylinositol 3-kinase signaling [ISO]
- positive regulation of protein catabolic process [ISO]
- progesterone receptor signaling pathway [ISO]
- protein K63-linked ubiquitination [IDA]
- protein monoubiquitination [ISO]
- protein targeting to lysosome [ISO]
- protein ubiquitination [ISO, ISS]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA, ISO]
- receptor catabolic process [ISO]
- receptor internalization [ISO]
- regulation of dendrite morphogenesis [IDA, ISO]
- regulation of ion transmembrane transport [ISO]
- regulation of membrane potential [ISO]
- regulation of potassium ion transmembrane transporter activity [ISO]
- regulation of synapse organization [ISO]
- response to denervation involved in regulation of muscle adaptation [IEP]
- transmission of virus [ISO]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [ISO]
Gene Ontology Molecular Function- RNA polymerase binding [ISO]
- beta-2 adrenergic receptor binding [ISO]
- phosphoserine binding [ISO]
- phosphothreonine binding [ISO]
- proline-rich region binding [ISO]
- protein C-terminus binding [IDA, IPI]
- protein binding [IPI]
- protein domain specific binding [ISO]
- sodium channel inhibitor activity [ISO]
- ubiquitin binding [ISO]
- ubiquitin protein ligase activity [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
- RNA polymerase binding [ISO]
- beta-2 adrenergic receptor binding [ISO]
- phosphoserine binding [ISO]
- phosphothreonine binding [ISO]
- proline-rich region binding [ISO]
- protein C-terminus binding [IDA, IPI]
- protein binding [IPI]
- protein domain specific binding [ISO]
- sodium channel inhibitor activity [ISO]
- ubiquitin binding [ISO]
- ubiquitin protein ligase activity [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Identification of Nedd4 E3 Ubiquitin Ligase as a Binding Partner and Regulator of MAK-V Protein Kinase.
MAK-V/Hunk is a scantily characterized AMPK-like protein kinase. Recent findings identified MAK-V as a pro-survival and anti-apoptotic protein and revealed its role in embryonic development as well as in tumorigenesis and metastasis. However molecular mechanisms of MAK-V action and regulation of its activity remain largely unknown. We identified Nedd4 as an interaction partner for MAK-V protein kinase. However, this HECT-type ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HUNK NEDD4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| NEDD4 HUNK | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID