BAIT

CRG1

YHR209W
S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
GO Process (1)
GO Function (3)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Saccharomyces cerevisiae (S288c)
PREY

ILM1

YJR118C
Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
GO Process (1)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

A systems biology approach reveals the role of a novel methyltransferase in response to chemical stress and lipid homeostasis.

Lissina E, Young B, Urbanus ML, Guan XL, Lowenson J, Hoon S, Baryshnikova A, Riezman I, Michaut M, Riezman H, Cowen LE, Wenk MR, Clarke SG, Giaever G, Nislow C

Using small molecule probes to understand gene function is an attractive approach that allows functional characterization of genes that are dispensable in standard laboratory conditions and provides insight into the mode of action of these compounds. Using chemogenomic assays we previously identified yeast Crg1, an uncharacterized SAM-dependent methyltransferase, as a novel interactor of the protein phosphatase inhibitor cantharidin. In this ... [more]

PLoS Genet. Sep. 01, 2011; 7(10);e1002332 [Pubmed: 22028670]

Throughput

  • High Throughput

Ontology Terms

  • vegetative growth (APO:0000106)
  • resistance to chemicals (APO:0000087)

Additional Notes

  • SGA with crg1 mutant as query strain, in the presence of 30 uM cantharidin

Curated By

  • BioGRID