An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

CGI-58 interacts with perilipin and is localized to lipid droplets. Possible involvement of CGI-58 mislocalization in Chanarin-Dorfman syndrome.

Yamaguchi T, Omatsu N, Matsushita S, Osumi T

Lipid droplets (LDs) are a class of ubiquitous cellular organelles that are involved in lipid storage and metabolism. Although the mechanisms of the biogenesis of LDs are still unclear, a set of proteins called the PAT domain family have been characterized as factors associating with LDs. Perilipin, a member of this family, is expressed exclusively in the adipose tissue and ... [more]

J. Biol. Chem. Jul. 16, 2004; 279(29);30490-7 [Pubmed: 15136565]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ABHD5

Gene Ontology Biological Process

Gene Ontology Molecular Function

lysophosphatidic acid acyltransferase activity [ISO]protein binding [IPI]triglyceride lipase activity [IDA]

Gene Ontology Cellular Component

PLIN2