BAIT

MARCH2

MARCH-II, RGD1306395
membrane-associated ring finger (C3HC4) 2, E3 ubiquitin protein ligase
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Rattus norvegicus
PREY

MARCH3

membrane-associated ring finger (C3HC4) 3, E3 ubiquitin protein ligase
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

MARCH-III Is a novel component of endosomes with properties similar to those of MARCH-II.

Fukuda H, Nakamura N, Hirose S

MARCH comprises a recently identified family of transmembrane RING-finger proteins which is implicated in diverse biological functions, such as immune regulation, protein quality control, and membrane trafficking. We previously identified MARCH-II, as a binding partner of syntaxin 6, which plays a role in endosomal protein transport. In this paper, we describe the cloning and characterization of MARCH-III which is the ... [more]

J. Biochem. Jan. 01, 2006; 139(1);137-45 [Pubmed: 16428329]

Throughput

  • Low Throughput

Curated By

  • BioGRID