BAIT
RAC1
ras-related C3 botulinum toxin substrate 1
GO Process (52)
GO Function (10)
GO Component (16)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISO]
- GTP catabolic process [IDA, ISO]
- Rac protein signal transduction [TAS]
- Wnt signaling pathway, planar cell polarity pathway [ISO]
- actin cytoskeleton organization [IDA, ISO]
- actin filament organization [IMP]
- actin filament polymerization [ISO]
- anatomical structure arrangement [ISO]
- auditory receptor cell morphogenesis [ISO]
- axon guidance [ISO]
- bone resorption [IDA]
- cell adhesion [ISO]
- cell migration [ISO]
- cell motility [ISO, ISS]
- cell proliferation [IMP]
- cell-cell junction organization [ISO]
- cellular component movement [IMP]
- cerebral cortex radially oriented cell migration [ISO]
- chemotaxis [IMP]
- cochlea morphogenesis [ISO]
- cytoskeleton organization [ISO]
- dendrite development [ISO]
- dendrite morphogenesis [ISO]
- dopaminergic neuron differentiation [ISO]
- embryonic olfactory bulb interneuron precursor migration [ISO]
- endocytosis [ISO]
- engulfment of apoptotic cell [ISO]
- epithelial cell morphogenesis [ISO]
- hyperosmotic response [ISO]
- lamellipodium assembly [ISO]
- localization within membrane [ISO]
- mast cell chemotaxis [IMP]
- negative regulation of interleukin-23 production [ISO]
- phagocytosis, engulfment [ISO]
- positive regulation of DNA replication [IMP]
- positive regulation of actin filament polymerization [ISO]
- positive regulation of cell-substrate adhesion [ISO]
- positive regulation of focal adhesion assembly [ISO]
- positive regulation of lamellipodium assembly [ISO]
- positive regulation of neutrophil chemotaxis [ISO]
- positive regulation of phosphatidylinositol 3-kinase activity [ISO]
- positive regulation of protein phosphorylation [ISO, ISS]
- positive regulation of stress fiber assembly [ISO]
- positive regulation of substrate adhesion-dependent cell spreading [ISO]
- protein localization to plasma membrane [ISO]
- regulation of cell migration [ISO, ISS]
- regulation of respiratory burst [ISO]
- ruffle assembly [ISO]
- ruffle organization [ISO]
- semaphorin-plexin signaling pathway [IMP]
- small GTPase mediated signal transduction [ISO]
- substrate adhesion-dependent cell spreading [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [IDA]
- actin filament [ISO]
- cell projection [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic membrane-bounded vesicle [ISO]
- cytoplasmic ribonucleoprotein granule [ISO]
- cytoplasmic vesicle [IDA]
- cytosol [IDA]
- extracellular vesicular exosome [ISO]
- extrinsic component of plasma membrane [ISO]
- focal adhesion [ISO]
- lamellipodium [ISO]
- membrane [IDA, ISO]
- phagocytic cup [ISO]
- ruffle membrane [ISO]
- trans-Golgi network [ISO]
Rattus norvegicus
PREY
PRKCD
Pkcd
protein kinase C, delta
GO Process (48)
GO Function (9)
GO Component (10)
Gene Ontology Biological Process
- B cell proliferation [ISO]
- aging [IEP]
- apoptotic process [ISO]
- aspartate transport [IMP]
- cellular response to glucose starvation [IEP]
- cellular response to insulin stimulus [IEP]
- cellular senescence [ISO]
- collagen metabolic process [IMP]
- defense response to bacterium [ISO, ISS]
- immunoglobulin mediated immune response [ISO]
- interleukin-10 production [ISO]
- interleukin-12 production [ISO]
- intracellular signal transduction [IDA]
- negative regulation of MAP kinase activity [ISO]
- negative regulation of actin filament polymerization [ISO, ISS]
- negative regulation of filopodium assembly [ISO, ISS]
- negative regulation of glial cell apoptotic process [ISO, ISS]
- negative regulation of insulin receptor signaling pathway [ISO]
- negative regulation of peptidyl-tyrosine phosphorylation [ISO]
- negative regulation of platelet aggregation [ISO, ISS]
- neutrophil activation [ISO]
- peptidyl-threonine phosphorylation [ISO]
- positive regulation of MAP kinase activity [IMP]
- positive regulation of MAPK cascade [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of apoptotic signaling pathway [ISO]
- positive regulation of ceramide biosynthetic process [ISO]
- positive regulation of glucose import [IMP]
- positive regulation of glucosylceramide catabolic process [ISO]
- positive regulation of phospholipid scramblase activity [ISO]
- positive regulation of protein dephosphorylation [ISO]
- positive regulation of response to DNA damage stimulus [ISO]
- positive regulation of sphingomyelin catabolic process [ISO]
- positive regulation of superoxide anion generation [ISO, ISS]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA, ISO]
- response to amino acid [IEP]
- response to drug [IEP]
- response to ethanol [IEP]
- response to glucose [IEP]
- response to heat [IEP]
- response to hydrogen peroxide [IEP]
- response to hypoxia [IEP]
- response to mechanical stimulus [IEP]
- response to organic cyclic compound [IEP]
- response to organonitrogen compound [IEP]
- response to oxidative stress [IMP]
- termination of signal transduction [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Celastrol binds to ERK and inhibits FcepsilonRI signaling to exert an anti-allergic effect.
The role of celastrol, a triterpene extracted from the Chinese "Thunder of God Vine," in allergic inflammation was investigated. Celastrol decreased the secretion of beta-hexosaminidase, decreased the release of histamine, decreased the expression of Th2 cytokines and decreased calcium influx and cell adhesion in antigen-stimulated RBL2H3 cells. Exposure to celastrol decreased the phosphorylation of extracellular regulated kinase (ERK) and the ... [more]
Eur. J. Pharmacol. Jun. 10, 2009; 612(1);131-42 [Pubmed: 19356729]
Throughput
- Low Throughput
Curated By
- BioGRID