VPS18
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VAMP8
Gene Ontology Biological Process
- SNARE complex assembly [IDA]
- autophagic vacuole fusion [ISO]
- exocytosis [IBA]
- membrane fusion [ISO]
- mucus secretion [IMP, ISO]
- negative regulation of secretion by cell [ISO]
- positive regulation of histamine secretion by mast cell [ISO]
- protein complex assembly [ISO]
- regulation of endocytosis [IMP]
- regulation of protein localization to plasma membrane [ISO]
- vesicle fusion [IBA, ISO]
- vesicle-mediated transport [ISO]
- viral entry into host cell [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IBA, ISO]
- azurophil granule membrane [ISO]
- cell surface [IDA]
- cytoplasm [ISO]
- cytosol [IDA, ISO]
- early endosome [TAS]
- extracellular vesicular exosome [ISO]
- late endosome membrane [ISO]
- lysosomal membrane [ISO]
- membrane [ISO]
- mitochondrion [IDA]
- mucin granule [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
- recycling endosome [ISO]
- secretory granule [IDA]
- secretory granule membrane [ISO]
- vesicle [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Mammalian late vacuole protein sorting orthologues participate in early endosomal fusion and interact with the cytoskeleton.
In Saccharomyces cerevisiae, the class C vacuole protein sorting (Vps) proteins, together with Vam2p/Vps41p and Vam6p/Vps39p, form a complex that interacts with soluble N-ethylmaleimide-sensitive factor attachment protein receptor and Rab proteins to "tether" vacuolar membranes before fusion. To determine a role for the corresponding mammalian orthologues, we examined the function, localization, and protein interactions of endogenous mVps11, mVps16, mVps18, mVam2p, ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID