BAIT

LASP1

AA408629, Def-4, SH3P6, Tg(Col1a1-lacZ)1Ngma, RP23-386E10.6
LIM and SH3 protein 1
GO Process (2)
GO Function (3)
GO Component (4)
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Phosphorylation of mouse LASP-1 on threonine 156 by cAMP- and cGMP-dependent protein kinase.

Keicher C, Gambaryan S, Schulze E, Marcus K, Meyer HE, Butt E

LIM and SH3 domain protein (LASP-1) is a specific focal adhesion protein involved in cell migration. Overlay studies demonstrate that LASP-1 directly binds to the proline-rich domains of zyxin, lipoma preferred partner (LPP), and vasodilator-stimulated phosphoprotein (VASP), with zyxin being the most prominent interacting partner. Despite the LIM/zinc-finger domain, hypothesized to be involved in homodimerization, LASP-1 exists as a monomer. ... [more]

Biochem. Biophys. Res. Commun. Nov. 05, 2004; 324(1);308-16 [Pubmed: 15465019]

Throughput

  • Low Throughput

Curated By

  • BioGRID