Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Differential ligand-dependent protein-protein interactions between nuclear receptors and a neuronal-specific cofactor.

Greiner EF, Kirfel J, Greschik H, Huang D, Becker P, Kapfhammer JP, Schuele R

Nuclear receptors are transcription factors that require multiple protein-protein interactions to regulate target gene expression. We have cloned a 27-kDa protein, termed NIX1 (neuronal interacting factor X 1), that directly binds nuclear receptors in vitro and in vivo. Protein-protein interaction between NIX1 and ligand-activated or constitutive active nuclear receptors, including retinoid-related orphan receptor beta (RORbeta) (NR1F2), strictly depends on the ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jun. 20, 2000; 97(13);7160-5 [Pubmed: 10860982]

Throughput

  • Low Throughput

Curated By

  • BioGRID