BAIT

NRIP2

AW491344, NIX1

nuclear receptor interacting protein 2

GO Process (1)

GO Function (1)

GO Component (2)

Gene Ontology Biological Process

negative regulation of transcription from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

cytoplasm [ISO]

CRISPR Database MGI Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

PREY

THRB

Nr1a2, T3R[b], T3Rbeta, Thrb1, Thrb2, c-erbAbeta

thyroid hormone receptor beta

GO Process (19)

GO Function (8)

GO Component (3)

Gene Ontology Biological Process

Type I pneumocyte differentiation [IGI]

female courtship behavior [IMP]

intracellular receptor signaling pathway [ISO, TAS]

negative regulation of female receptivity [IMP]

negative regulation of transcription from RNA polymerase II promoter [IGI]

negative regulation of transcription, DNA-templated [IDA]

organ morphogenesis [IMP]

positive regulation of cell proliferation [ISO]

positive regulation of chondrocyte differentiation [ISO]

positive regulation of ossification [ISO]

positive regulation of transcription from RNA polymerase II promoter [IGI]

regulation of cholesterol metabolic process [ISO]

regulation of heart contraction [IMP]

regulation of lipid metabolic process [ISO]

regulation of transcription from RNA polymerase II promoter [IGI, ISO]

regulation of transcription, DNA-templated [TAS]

regulation of triglyceride metabolic process [ISO]

sensory perception of sound [IMP]

transcription from RNA polymerase II promoter [ISO]

Gene Ontology Molecular Function

chromatin DNA binding [IDA]
double-stranded DNA binding [ISO]
enzyme binding [ISO]
protein binding [IPI]
protein homodimerization activity [ISO]
sequence-specific DNA binding transcription factor activity [TAS]
thyroid hormone binding [ISO]
thyroid hormone receptor activity [ISO, TAS]

Gene Ontology Cellular Component

extracellular vesicular exosome [ISO]

nuclear chromatin [IDA]

nucleus [IDA, TAS]

CRISPR Database MGI Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Differential ligand-dependent protein-protein interactions between nuclear receptors and a neuronal-specific cofactor.

Greiner EF, Kirfel J, Greschik H, Huang D, Becker P, Kapfhammer JP, Schuele R

Nuclear receptors are transcription factors that require multiple protein-protein interactions to regulate target gene expression. We have cloned a 27-kDa protein, termed NIX1 (neuronal interacting factor X 1), that directly binds nuclear receptors in vitro and in vivo. Protein-protein interaction between NIX1 and ligand-activated or constitutive active nuclear receptors, including retinoid-related orphan receptor beta (RORbeta) (NR1F2), strictly depends on the ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jun. 20, 2000; 97(13);7160-5 [Pubmed: 10860982]

Throughput

Low Throughput

Curated By

BioGRID