Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

RFPL4 interacts with oocyte proteins of the ubiquitin-proteasome degradation pathway.

Suzumori N, Burns KH, Yan W, Matzuk MM

Oocyte meiosis and early mitotic divisions in developing embryos rely on the timely production of cell cycle regulators and their clearance via proteasomal degradation. Ret Finger Protein-Like 4 (Rfpl4), encoding a RING finger-like protein with a B30.2 domain, was discovered during an in silico search for germ cell-specific genes. To study the expression and functions of RFPL4 protein, we performed ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jan. 21, 2003; 100(2);550-5 [Pubmed: 12525704]

Throughput

  • Low Throughput

Curated By

  • BioGRID