PREY

AR

AIS, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM, RP11-383C12.1
androgen receptor
GO Process (23)
GO Function (16)
GO Component (5)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

APPL suppresses androgen receptor transactivation via potentiating Akt activity.

Yang L, Lin HK, Altuwaijri S, Xie S, Wang L, Chang C

APPL may function as an adapter protein to modulate the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. Although we have previously proven that the PI3K/Akt pathway can suppress androgen receptor (AR) transactivation, the potential linkage from APPL to the AR remains unclear. Here we demonstrated that APPL could suppress AR-mediated transactivation in a dose-dependent manner in LNCaP and PC-3 cells. This suppressive effect ... [more]

J. Biol. Chem. May. 09, 2003; 278(19);16820-7 [Pubmed: 12621049]

Throughput

  • Low Throughput

Additional Notes

  • figure 8.

Curated By

  • BioGRID