An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

CIR, a corepressor of CBF1, binds to PAP-1 and effects alternative splicing.

Maita H, Kitaura H, Ariga H, Iguchi-Ariga SM

We have reported that PAP-1, a product of a causative gene for autosomal retinitis pigmentosa, plays a role in splicing. In this study, CIR, a protein originally identified as a CBF1-interacting protein and reported to act as a transcriptional corepressor, was identified as a PAP-1 binding protein and its function as a splicing factor was investigated. In addition to a ... [more]

Exp. Cell Res. Feb. 15, 2005; 303(2);375-87 [Pubmed: 15652350]

Throughput

Low Throughput

Additional Notes

figure 2.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RP9 CIR1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	1	BioGRID	3080929
CIR1 RP9	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Maita H (2005)	Low	-	BioGRID	853654
RP9 CIR1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Maita H (2005)	Low	-	BioGRID	853658
CIR1 RP9	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Maita H (2005)	Low	-	BioGRID	853659

Curated By

BioGRID

Interaction Summary

RP9

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

CIR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]protein binding [IPI]sequence-specific DNA binding transcription factor activity [TAS]transcription corepressor activity [TAS]