CANX
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ER-mitochondrion membrane contact site [ISO]
- axon [ISO]
- cell [IMP]
- cytoplasm [ISO]
- dendrite cytoplasm [ISO]
- dendritic spine [ISO]
- endoplasmic reticulum [IDA, ISO]
- endoplasmic reticulum membrane [ISO]
- integral component of membrane [IDA]
- membrane [IDA, ISO]
- myelin sheath [IDA]
- neuronal cell body [ISO]
- protein complex [ISO]
- ribosome [ISO]
- rough endoplasmic reticulum [ISO]
- smooth endoplasmic reticulum [ISO]
CHRNB1
Gene Ontology Biological Process
- behavioral response to nicotine [ISO]
- cation transmembrane transport [IGI, ISO]
- cation transport [ISO]
- ion transmembrane transport [ISO]
- muscle contraction [ISO]
- muscle fiber development [ISO]
- neurological system process [ISO]
- neuromuscular synaptic transmission [ISO]
- postsynaptic membrane organization [ISO]
- regulation of membrane potential [IGI]
- signal transduction [ISO]
- skeletal muscle contraction [ISO]
- synaptic transmission, cholinergic [ISO]
- transmembrane transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Involvement of the chaperone protein calnexin and the acetylcholine receptor beta-subunit in the assembly and cell surface expression of the receptor.
The nicotinic acetylcholine receptor at the neuromuscular junction is a ligand-gated ion channel assembled in the endoplasmic reticulum from four distinct glycoprotein subunits into the pentameric configuration of alpha2betagammadelta. The individual homologous subunits form specific contacts at interfaces with neighboring subunits to achieve the appropriate orientation and order of each subunit in surrounding the ion channel. Assembly is thought to ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID