BAIT

CORO1A

Clabp, Lmb3, TACO, p57
coronin, actin binding protein 1A
GO Process (26)
GO Function (8)
GO Component (18)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Association of the leukocyte plasma membrane with the actin cytoskeleton through coiled coil-mediated trimeric coronin 1 molecules.

Gatfield J, Albrecht I, Zanolari B, Steinmetz MO, Pieters J

Coronin 1 is a member of the coronin protein family specifically expressed in leukocytes and accumulates at sites of rearrangements of the F-actin cytoskeleton. Here, we describe that coronin 1 molecules are coiled coil-mediated homotrimeric complexes, which associate with the plasma membrane and with the cytoskeleton via two distinct domains. Association with the cytoskeleton was mediated by trimerization of a ... [more]

Mol. Biol. Cell Jun. 01, 2005; 16(6);2786-98 [Pubmed: 15800061]

Throughput

  • Low Throughput

Curated By

  • BioGRID