VAMP2
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [ISO]
- calcium ion-dependent exocytosis [ISO]
- cellular response to insulin stimulus [ISO]
- eosinophil degranulation [ISO]
- long-term synaptic potentiation [ISO]
- membrane fusion [ISO]
- mucus secretion [ISO]
- natural killer cell degranulation [ISO]
- neurotransmitter secretion [IBA]
- neutrophil degranulation [ISO]
- positive regulation of intracellular protein transport [ISO]
- protein complex assembly [IDA]
- protein transport [IMP]
- regulation of exocytosis [IMP, ISO, TAS]
- regulation of histamine secretion by mast cell [ISO]
- regulation of vesicle-mediated transport [ISO]
- response to glucose [IDA]
- synaptic vesicle exocytosis [ISO]
- vesicle fusion [IBA]
- vesicle-mediated transport [IMP]
Gene Ontology Molecular Function- SNAP receptor activity [IBA]
- SNARE binding [IDA, ISO]
- calcium-dependent protein binding [IPI]
- calmodulin binding [ISO]
- identical protein binding [IPI]
- myosin binding [IPI]
- phospholipid binding [ISO]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- syntaxin binding [ISO]
- syntaxin-1 binding [IDA, IPI]
- SNAP receptor activity [IBA]
- SNARE binding [IDA, ISO]
- calcium-dependent protein binding [IPI]
- calmodulin binding [ISO]
- identical protein binding [IPI]
- myosin binding [IPI]
- phospholipid binding [ISO]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- syntaxin binding [ISO]
- syntaxin-1 binding [IDA, IPI]
Gene Ontology Cellular Component
- SNARE complex [IDA, ISO]
- cell [ISO]
- clathrin-coated vesicle [ISO, ISS]
- cytoplasmic vesicle [ISO]
- extracellular vesicular exosome [ISO]
- intracellular [ISO]
- intracellular membrane-bounded organelle [IDA]
- intracellular organelle [IDA]
- membrane [ISO, ISS]
- neuron projection [IDA]
- neuron projection terminus [IDA]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- secretory granule [IDA, ISO]
- secretory granule membrane [ISO]
- synapse [ISO]
- synaptic vesicle [IBA, IDA, ISO]
- synaptic vesicle membrane [ISO, NAS]
- synaptobrevin 2-SNAP-25-syntaxin-1a complex [IDA, ISO]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex [IDA]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex [IDA]
- terminal bouton [IDA]
- trans-Golgi network [ISO]
- zymogen granule membrane [ISO]
SNAP25
Gene Ontology Biological Process
- calcium ion-dependent exocytosis of neurotransmitter [ISO]
- endosomal transport [ISO]
- long-term memory [ISO]
- long-term synaptic potentiation [IMP]
- neurotransmitter secretion [IMP]
- positive regulation of hormone secretion [ISO]
- potassium ion transmembrane transport [ISO]
- regulation of establishment of protein localization [IMP]
- regulation of ion transmembrane transport [ISO]
- regulation of neuron projection development [ISO]
- sleep [ISO]
- synaptic vesicle fusion to presynaptic membrane [IBA]
- synaptic vesicle priming [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- BLOC-1 complex [ISO]
- SNARE complex [ISO]
- actin cytoskeleton [ISO]
- axon [ISO]
- axonal growth cone [ISO]
- cell [IMP]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- endosome [ISO]
- filopodium [ISO]
- growth cone [ISO]
- intracellular organelle [ISO]
- lamellipodium [ISO]
- membrane [IDA]
- membrane raft [ISO]
- myelin sheath [IDA]
- neuron projection [ISO]
- neuronal cell body [ISO]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [ISO, TAS]
- synapse [IDA]
- synaptobrevin 2-SNAP-25-syntaxin-1a complex [ISO]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex [IDA, ISO]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex [ISO]
- terminal bouton [ISO]
- trans-Golgi network [IDA]
- vesicle [ISO]
- voltage-gated potassium channel complex [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Distinct protein domains are responsible for the interaction of Hrs-2 with SNAP-25. The role of Hrs-2 in 7 S complex formation.
Regulated secretion of neurotransmitter at the synapse is likely to be mediated by dynamic protein interactions involving components of the vesicle (vesicle-associated membrane protein; VAMP) and plasma membrane (syntaxin and synaptosomal associated protein of 25 kDa (SNAP-25)) along with additional molecules that allow for the regulation of this process. Recombinant Hrs-2 interacts with SNAP-25 in a calcium-dependent manner (they dissociate ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID