BAIT
CAV3
caveolin 3
GO Process (56)
GO Function (12)
GO Component (17)
Gene Ontology Biological Process
- actin filament organization [ISO]
- cardiac muscle cell development [ISO]
- caveola assembly [ISO]
- cell differentiation [IEP]
- cell growth [IEP]
- cholesterol homeostasis [ISO]
- cytoplasmic microtubule organization [ISO]
- endocytosis [ISO]
- establishment of protein localization to plasma membrane [ISO]
- glucose homeostasis [ISO]
- heart trabecula formation [ISO]
- membrane raft organization [ISO]
- muscle cell cellular homeostasis [ISO]
- myoblast fusion [ISO]
- myotube differentiation [ISO]
- negative regulation of MAP kinase activity [ISO]
- negative regulation of MAPK cascade [ISO]
- negative regulation of calcium ion transport [ISO]
- negative regulation of cardiac muscle hypertrophy [ISO]
- negative regulation of cell growth involved in cardiac muscle cell development [IMP]
- negative regulation of cell size [ISO]
- negative regulation of nitric-oxide synthase activity [ISO]
- negative regulation of potassium ion transmembrane transport [IMP]
- negative regulation of potassium ion transmembrane transporter activity [IMP]
- negative regulation of protein kinase activity [ISO]
- negative regulation of sarcomere organization [ISO]
- nucleus localization [ISO]
- plasma membrane organization [ISO]
- plasma membrane repair [ISO]
- positive regulation of caveolin-mediated endocytosis [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of cytosolic calcium ion concentration [IMP]
- positive regulation of microtubule polymerization [IMP]
- positive regulation of myotube differentiation [ISO]
- protein localization [ISO]
- protein localization to plasma membrane [ISO]
- regulation of branching involved in mammary gland duct morphogenesis [ISO]
- regulation of calcium ion import [ISO]
- regulation of calcium ion transmembrane transporter activity [ISO]
- regulation of calcium ion transport [ISO]
- regulation of cardiac muscle contraction [ISO]
- regulation of heart contraction [IMP]
- regulation of heart rate [ISO]
- regulation of membrane depolarization during cardiac muscle cell action potential [ISO]
- regulation of membrane potential [ISO]
- regulation of nerve growth factor receptor activity [ISO]
- regulation of p38MAPK cascade [ISO]
- regulation of protein kinase B signaling [ISO]
- regulation of signal transduction by receptor internalization [ISO]
- regulation of skeletal muscle contraction [ISO]
- regulation of sodium ion transmembrane transporter activity [ISO]
- regulation of transforming growth factor beta receptor signaling pathway [ISO]
- regulation of ventricular cardiac muscle cell membrane depolarization [ISO]
- regulation of ventricular cardiac muscle cell membrane repolarization [ISO]
- triglyceride metabolic process [ISO]
- ventricular cardiac muscle cell action potential [IMP]
Gene Ontology Molecular Function- alpha-tubulin binding [ISO]
- calcium channel regulator activity [ISO]
- connexin binding [ISO]
- enzyme binding [IPI]
- ion channel binding [IPI, ISO]
- nitric-oxide synthase binding [IPI]
- potassium channel inhibitor activity [IMP]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein complex scaffold [IDA, ISO]
- sodium channel regulator activity [ISO]
- alpha-tubulin binding [ISO]
- calcium channel regulator activity [ISO]
- connexin binding [ISO]
- enzyme binding [IPI]
- ion channel binding [IPI, ISO]
- nitric-oxide synthase binding [IPI]
- potassium channel inhibitor activity [IMP]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein complex scaffold [IDA, ISO]
- sodium channel regulator activity [ISO]
Gene Ontology Cellular Component
- T-tubule [IDA, ISO]
- Z disc [IDA]
- caveola [IDA, ISO]
- cell [ISO]
- cell surface [IDA]
- cytoplasm [IDA]
- dystrophin-associated glycoprotein complex [ISO]
- endoplasmic reticulum [ISO]
- integral component of membrane [TAS]
- intercalated disc [IDA]
- membrane [ISO]
- membrane raft [ISO]
- neuromuscular junction [IDA]
- plasma membrane [IDA, ISO]
- protein complex [IDA]
- sarcolemma [IDA, ISO]
- vesicle [IDA]
Rattus norvegicus
PREY
DLG1
Dlgh1, SAP97
discs, large homolog 1 (Drosophila)
GO Process (34)
GO Function (11)
GO Component (30)
Gene Ontology Biological Process
- T cell activation [ISO]
- T cell cytokine production [ISO]
- actin filament organization [ISO, ISS]
- activation of protein kinase activity [ISO]
- amyloid precursor protein metabolic process [ISO]
- branching involved in ureteric bud morphogenesis [ISO]
- cell adhesion [IEP]
- cortical actin cytoskeleton organization [ISO, ISS]
- embryonic skeletal system morphogenesis [ISO]
- endothelial cell proliferation [ISO, ISS]
- hard palate development [ISO]
- immunological synapse formation [ISO]
- lens development in camera-type eye [ISO]
- membrane raft organization [ISO]
- negative regulation of T cell proliferation [ISO]
- negative regulation of epithelial cell proliferation [ISO]
- negative regulation of mitotic cell cycle [ISO, ISS]
- negative regulation of protein kinase B signaling [ISO]
- peristalsis [ISO]
- positive regulation of actin filament polymerization [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of establishment of protein localization to plasma membrane [ISO]
- positive regulation of potassium ion transport [IMP, ISO]
- protein localization [ISO]
- protein localization to plasma membrane [ISO]
- regulation of membrane potential [ISO]
- regulation of myelination [ISO]
- regulation of protein localization [IMP]
- reproductive structure development [ISO]
- single organismal cell-cell adhesion [ISO, ISS]
- smooth muscle tissue development [ISO]
- tight junction assembly [ISO]
- tissue morphogenesis [ISO]
- ureteric bud development [ISO]
Gene Ontology Molecular Function- L27 domain binding [ISO]
- PDZ domain binding [IPI]
- ion channel binding [ISO]
- ionotropic glutamate receptor binding [IPI]
- mitogen-activated protein kinase kinase binding [ISO]
- phosphatase binding [ISO, ISS]
- potassium channel regulator activity [ISO]
- protein C-terminus binding [IPI, ISO]
- protein binding [IPI]
- protein complex scaffold [ISO]
- protein kinase binding [IPI, ISO, ISS]
- L27 domain binding [ISO]
- PDZ domain binding [IPI]
- ion channel binding [ISO]
- ionotropic glutamate receptor binding [IPI]
- mitogen-activated protein kinase kinase binding [ISO]
- phosphatase binding [ISO, ISS]
- potassium channel regulator activity [ISO]
- protein C-terminus binding [IPI, ISO]
- protein binding [IPI]
- protein complex scaffold [ISO]
- protein kinase binding [IPI, ISO, ISS]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- MPP7-DLG1-LIN7 complex [ISO]
- T-tubule [IDA]
- basal lamina [ISO]
- basal plasma membrane [IDA]
- basolateral plasma membrane [IDA, ISO, ISS]
- cell junction [ISO]
- cell projection membrane [ISO]
- cell-cell junction [ISO]
- cytoplasm [ISO]
- cytoplasmic side of plasma membrane [ISO]
- endoplasmic reticulum [ISO]
- extracellular vesicular exosome [ISO]
- immunological synapse [ISO]
- lateral loop [ISO]
- lateral plasma membrane [ISO]
- membrane raft [ISO]
- microtubule [ISO]
- myelin sheath abaxonal region [ISO]
- neuromuscular junction [ISO]
- neuron projection [IDA]
- node of Ranvier [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [IDA, ISO, TAS]
- postsynaptic membrane [TAS]
- presynaptic membrane [IDA]
- synapse [ISO]
- tight junction [ISO]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Caveolin-3 and SAP97 form a scaffolding protein complex that regulates the voltage-gated potassium channel Kv1.5.
The targeting of ion channels to particular membrane microdomains and their organization in macromolecular complexes allow excitable cells to respond efficiently to extracellular signals. In this study, we describe the formation of a complex that contains two scaffolding proteins: caveolin-3 (Cav-3) and a membrane-associated guanylate kinase (MAGUK), SAP97. Complex formation involves the association of Cav-3 with a segment of SAP97 ... [more]
Am. J. Physiol. Heart Circ. Physiol. Aug. 01, 2004; 287(2);H681-90 [Pubmed: 15277200]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DLG1 CAV3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID