Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Identification of Atrogin-1 targeted proteins during the Myostatin-induced skeletal muscle wasting.

Lokireddy S, Wijesoma IW, Sze SK, McFarlane C, Kambadur R, Sharma M

Atrogin-1, a muscle-specific E3 ligase, targets MyoD for degradation through the ubiquitin-proteasome mediated system. Myostatin, a member of the TGF-β superfamily, potently inhibits myogenesis by lowering MyoD levels. While Atrogin-1 is up-regulated by Myostatin, it is currently unknown whether Atrogin-1 plays a role in mediating Myostatin signaling to regulate myogenesis. In this report, we have confirmed that Atrogin-1 increasingly interacts ... [more]

Unknown Jun. 06, 2012; 0(0); [Pubmed: 22673621]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FBXO32 DES
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
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Curated By

  • BioGRID