PREY

MYB

AI550390, M16449, c-myb
myeloblastosis oncogene
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Pin1 interacts with c-Myb in a phosphorylation-dependent manner and regulates its transactivation activity.

Pani E, Menigatti M, Schubert S, Hess D, Gerrits B, Klempnauer KH, Ferrari S

Activity and stability of the proto-oncogene c-Myb are regulated by post-translational modifications, though the molecular mechanisms underlying such control are only partially understood. Here we describe the functional interaction of c-Myb with Pin1, an isomerase that binds to phosphorylated Ser/Thr-Pro motifs. We found that co-expression of c-Myb and Pin1 led to a net increase of c-Myb transactivation activity, both on ... [more]

Biochim. Biophys. Acta Jun. 01, 2008; 1783(6);1121-8 [Pubmed: 18359295]

Throughput

  • Low Throughput

Additional Notes

  • figure 3.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MYB PIN1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
854126

Curated By

  • BioGRID