Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Bag1-L is a phosphorylation-dependent coactivator of c-Jun during neuronal apoptosis.

Da Costa CR, Villadiego J, Sancho R, Fontana X, Packham G, Nateri AS, Behrens A

In the nervous system, cell death by apoptosis plays a critical role during normal development and pathological neurodegeneration. Jun N-terminal kinases (JNKs) are essential regulators of neuronal apoptosis. The AP-1 transcription factor c-Jun is phosphorylated at multiple sites within its transactivation domain by the JNKs, and c-Jun phosphorylation is required for JNK-induced neurotoxicity. While the importance of c-Jun as a ... [more]

Mol. Cell. Biol. Aug. 01, 2010; 30(15);3842-52 [Pubmed: 20516211]

Throughput

  • Low Throughput

Curated By

  • BioGRID