BAIT

PRMT2

AI504737, Hrmt1l1
protein arginine N-methyltransferase 2
GO Process (16)
GO Function (14)
GO Component (5)

Gene Ontology Cellular Component

Mus musculus
PREY

RB1

Rb, Rb-1, pRb
retinoblastoma 1
GO Process (36)
GO Function (9)
GO Component (5)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The arginine methyltransferase PRMT2 binds RB and regulates E2F function.

Yoshimoto T, Boehm M, Olive M, Crook MF, San H, Langenickel T, Nabel EG

The retinoblastoma gene product (RB) is an important regulator of E2F activity. RB recruits a number of proteins, including HDACs, SWI/SNF complex, lysine methyl transferase (SUV39H1) and DNA methyltransferase (DNMT1), all of which negatively regulate E2F activity with RB. Here, we show that RB interacts with PRMT2, a member of the protein arginine methyltransferase family, to regulate E2F activity. PRMT2 ... [more]

Exp. Cell Res. Jul. 01, 2006; 312(11);2040-53 [Pubmed: 16616919]

Throughput

  • Low Throughput

Curated By

  • BioGRID