BAIT

KRAS

AI929937, K-ras, Ki-ras, Kras-2, Kras2, p21B, ras, RP24-359O2.1
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Inactivation of Icmt inhibits transformation by oncogenic K-Ras and B-Raf.

Bergo MO, Gavino BJ, Hong C, Beigneux AP, McMahon M, Casey PJ, Young SG

Isoprenylcysteine carboxyl methyltransferase (Icmt) methylates the carboxyl-terminal isoprenylcysteine of CAAX proteins (e.g., Ras and Rho proteins). In the case of the Ras proteins, carboxyl methylation is important for targeting of the proteins to the plasma membrane. We hypothesized that a knockout of Icmt would reduce the ability of cells to be transformed by K-Ras. Fibroblasts harboring a floxed Icmt allele ... [more]

J. Clin. Invest. Feb. 01, 2004; 113(4);539-50 [Pubmed: 14966563]

Throughput

  • Low Throughput

Curated By

  • BioGRID