BAIT
PSEN1
Ad3h, PS-1, PS1, S182
presenilin 1
GO Process (86)
GO Function (7)
GO Component (35)
Gene Ontology Biological Process
- Cajal-Retzius cell differentiation [IMP]
- L-glutamate transport [IMP]
- Notch receptor processing [IMP]
- Notch signaling pathway [IGI, IMP]
- T cell activation involved in immune response [IGI]
- T cell receptor signaling pathway [IGI]
- activation of MAPKK activity [IMP]
- amyloid precursor protein catabolic process [IMP]
- anagen [IGI]
- autophagic vacuole assembly [IMP]
- autophagy [IMP]
- beta-amyloid formation [IMP]
- beta-amyloid metabolic process [IMP]
- blood vessel development [IMP]
- brain development [IMP]
- brain morphogenesis [IGI]
- calcium ion transmembrane transport [ISO]
- calcium ion transport [IBA]
- canonical Wnt signaling pathway [IBA]
- cell fate specification [IGI]
- cellular calcium ion homeostasis [IMP]
- cellular protein metabolic process [IDA, IGI, IMP]
- cellular response to DNA damage stimulus [IMP]
- cerebral cortex cell migration [IMP]
- cerebral cortex development [IMP]
- choline transport [IMP]
- dorsal/ventral neural tube patterning [IGI]
- embryo development [IGI, IMP]
- embryonic limb morphogenesis [IGI]
- endoplasmic reticulum calcium ion homeostasis [IGI, IMP, ISO]
- epithelial cell proliferation [IGI]
- forebrain development [IGI]
- heart development [IMP]
- heart looping [IGI]
- hematopoietic progenitor cell differentiation [IGI]
- learning or memory [IGI]
- locomotion [IGI]
- membrane protein ectodomain proteolysis [IBA, ISO]
- memory [IGI, IMP]
- mitochondrial transport [IMP]
- myeloid dendritic cell differentiation [IMP]
- myeloid leukocyte differentiation [IGI]
- negative regulation of apoptotic process [IDA, ISO]
- negative regulation of apoptotic signaling pathway [IGI]
- negative regulation of axonogenesis [IMP]
- negative regulation of epidermal growth factor-activated receptor activity [IMP]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of protein kinase activity [IMP]
- negative regulation of protein phosphorylation [IGI]
- negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of ubiquitin-protein transferase activity [IMP]
- neurogenesis [IMP]
- neuron apoptotic process [IGI]
- neuron development [IMP]
- neuron differentiation [IMP]
- neuron migration [IMP]
- positive regulation of MAP kinase activity [IMP]
- positive regulation of apoptotic process [IGI]
- positive regulation of catalytic activity [ISO]
- positive regulation of coagulation [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- positive regulation of protein kinase activity [IDA]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of receptor recycling [IMP]
- post-embryonic development [IMP]
- protein glycosylation [IMP]
- protein maturation [IGI]
- protein processing [IMP, ISO]
- protein transport [IGI]
- proteolysis [IGI]
- regulation of epidermal growth factor-activated receptor activity [IGI]
- regulation of phosphorylation [ISO]
- regulation of protein binding [IGI]
- regulation of resting membrane potential [IMP]
- regulation of synaptic plasticity [IGI, IMP]
- regulation of synaptic transmission, glutamatergic [IMP]
- response to oxidative stress [IGI, IMP]
- segmentation [IMP]
- single organismal cell-cell adhesion [ISO]
- skeletal system morphogenesis [IMP]
- skin morphogenesis [IMP]
- smooth endoplasmic reticulum calcium ion homeostasis [IGI]
- somitogenesis [IGI, IMP]
- synaptic vesicle targeting [IMP]
- thymus development [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA, ISO]
- Z disc [IBA]
- apical plasma membrane [IBA]
- axon [IDA]
- cell [IGI]
- cell cortex [IBA]
- cell surface [IBA, ISO]
- centrosome [ISO]
- ciliary rootlet [IDA]
- cytoplasmic vesicle [IDA]
- dendrite [IDA]
- dendritic shaft [IDA]
- endoplasmic reticulum [IDA, IMP, ISO]
- gamma-secretase complex [ISO]
- growth cone [IDA]
- integral component of plasma membrane [IBA, ISO]
- intracellular [IDA, IMP]
- kinetochore [ISO]
- lysosomal membrane [IBA, ISO]
- membrane [IDA]
- membrane raft [IBA, ISO]
- membrane-bounded organelle [IDA]
- mitochondrial inner membrane [IBA, ISO]
- mitochondrion [ISO]
- neuromuscular junction [IBA, ISO]
- neuron projection [ISO]
- neuronal cell body [IDA]
- nuclear membrane [ISO]
- nuclear outer membrane [ISO]
- nucleus [IDA]
- perinuclear region of cytoplasm [IBA]
- plasma membrane [IDA]
- protein complex [ISO]
- rough endoplasmic reticulum [ISO]
- smooth endoplasmic reticulum [IGI, ISO]
Mus musculus
PREY
GRIN2A
GluN2A, NMDAR2A, NR2A
glutamate receptor, ionotropic, NMDA2A (epsilon 1)
GO Process (44)
GO Function (19)
GO Component (13)
Gene Ontology Biological Process
- action potential [ISO]
- calcium ion transmembrane transport [IMP]
- calcium ion transport [IGI, IMP]
- cation transmembrane transport [ISO]
- cation transport [ISO]
- cellular response to amino acid stimulus [ISO]
- cellular response to zinc ion [ISO]
- detection of mechanical stimulus involved in sensory perception of pain [IMP]
- directional locomotion [IGI]
- dopamine metabolic process [IMP]
- ion transmembrane transport [IGI, ISO]
- ionotropic glutamate receptor signaling pathway [IGI, ISO]
- learning [IMP]
- learning or memory [IMP]
- locomotion [IMP]
- locomotory behavior [TAS]
- memory [IMP, ISO]
- negative regulation of protein catabolic process [IGI]
- neurogenesis [IMP]
- positive regulation of apoptotic process [IGI]
- positive regulation of cell death [ISO]
- positive regulation of excitatory postsynaptic membrane potential [ISO]
- protein localization [IGI, IMP]
- protein tetramerization [ISO]
- regulation of excitatory postsynaptic membrane potential [IGI, IMP, ISO]
- regulation of ion transmembrane transport [ISO]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of membrane potential [IMP]
- regulation of postsynaptic membrane potential [IMP]
- regulation of sensory perception of pain [IMP]
- regulation of synaptic plasticity [IMP]
- regulation of synaptic transmission [IMP]
- response to amphetamine [IMP]
- response to drug [IMP]
- response to ethanol [IMP, ISO]
- response to wounding [IGI, IMP]
- rhythmic process [ISO]
- sensory perception of pain [IMP]
- serotonin metabolic process [IMP]
- sleep [IMP]
- startle response [IGI, IMP]
- synaptic transmission [IMP, ISO]
- synaptic transmission, glutamatergic [IBA]
- visual learning [IMP]
Gene Ontology Molecular Function- ATPase binding [ISO]
- N-methyl-D-aspartate selective glutamate receptor activity [IGI, IMP, ISO]
- calcium channel activity [IGI, IMP]
- cation channel activity [IMP, ISO]
- cell adhesion molecule binding [ISO]
- extracellular-glutamate-gated ion channel activity [IBA, ISO]
- glutamate binding [ISO]
- glutamate receptor binding [ISO]
- ionotropic glutamate receptor activity [ISO]
- neurotransmitter binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein dimerization activity [ISO]
- protein heterodimerization activity [ISO]
- protein kinase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [ISO]
- voltage-gated cation channel activity [ISO]
- zinc ion binding [ISO]
- ATPase binding [ISO]
- N-methyl-D-aspartate selective glutamate receptor activity [IGI, IMP, ISO]
- calcium channel activity [IGI, IMP]
- cation channel activity [IMP, ISO]
- cell adhesion molecule binding [ISO]
- extracellular-glutamate-gated ion channel activity [IBA, ISO]
- glutamate binding [ISO]
- glutamate receptor binding [ISO]
- ionotropic glutamate receptor activity [ISO]
- neurotransmitter binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein dimerization activity [ISO]
- protein heterodimerization activity [ISO]
- protein kinase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [ISO]
- voltage-gated cation channel activity [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
- N-methyl-D-aspartate selective glutamate receptor complex [IPI, ISO]
- cell surface [IDA]
- dendritic spine [ISA]
- endoplasmic reticulum [IDA]
- membrane [IDA]
- neuron projection [IDA, ISO]
- neuronal postsynaptic density [IDA]
- postsynaptic density [IDA, ISO]
- postsynaptic membrane [IDA]
- presynaptic membrane [IDA, ISO]
- synapse [IDA]
- synaptic vesicle [IDA]
- terminal bouton [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Loss of presenilin function causes impairments of memory and synaptic plasticity followed by age-dependent neurodegeneration.
Mutations in presenilins are the major cause of familial Alzheimer's disease, but the pathogenic mechanism by which presenilin mutations cause memory loss and neurodegeneration remains unclear. Here we demonstrate that conditional double knockout mice lacking both presenilins in the postnatal forebrain exhibit impairments in hippocampal memory and synaptic plasticity. These deficits are associated with specific reductions in NMDA receptor-mediated responses ... [more]
Neuron Apr. 08, 2004; 42(1);23-36 [Pubmed: 15066262]
Throughput
- Low Throughput
Curated By
- BioGRID