NOS2
Gene Ontology Biological Process
- arginine catabolic process [IDA, ISO]
- cellular response to interferon-gamma [IDA]
- cellular response to lipopolysaccharide [IDA]
- defense response to bacterium [IMP, ISO]
- inflammatory response [TAS]
- negative regulation of blood pressure [IBA]
- negative regulation of gene expression [IGI, ISO]
- negative regulation of protein catabolic process [IDA]
- nitric oxide biosynthetic process [IDA, ISO]
- nitric oxide mediated signal transduction [IBA]
- ovulation from ovarian follicle [IMP]
- oxidation-reduction process [IBA]
- peptidyl-cysteine S-nitrosylation [IDA]
- positive regulation of guanylate cyclase activity [IBA]
- positive regulation of killing of cells of other organism [ISO]
- positive regulation of vasodilation [IBA]
- regulation of cell proliferation [IGI]
- regulation of insulin secretion [ISO]
- regulation of protein kinase activity [ISO]
- response to hypoxia [IDA]
- response to lipopolysaccharide [IDA]
- superoxide metabolic process [IMP]
Gene Ontology Molecular Function- FMN binding [IDA]
- Hsp90 protein binding [ISO]
- NADPH-hemoprotein reductase activity [IBA]
- actin binding [ISO]
- arginine binding [IDA]
- beta-catenin binding [ISO]
- cAMP-dependent protein kinase regulator activity [ISO]
- cadherin binding [ISO]
- calmodulin binding [TAS]
- flavin adenine dinucleotide binding [IDA]
- heme binding [IDA]
- nitric-oxide synthase activity [IDA, ISO]
- nitric-oxide synthase binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [IDA, ISO]
- protein kinase binding [ISO]
- receptor binding [ISO]
- tetrahydrobiopterin binding [IDA]
- FMN binding [IDA]
- Hsp90 protein binding [ISO]
- NADPH-hemoprotein reductase activity [IBA]
- actin binding [ISO]
- arginine binding [IDA]
- beta-catenin binding [ISO]
- cAMP-dependent protein kinase regulator activity [ISO]
- cadherin binding [ISO]
- calmodulin binding [TAS]
- flavin adenine dinucleotide binding [IDA]
- heme binding [IDA]
- nitric-oxide synthase activity [IDA, ISO]
- nitric-oxide synthase binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [IDA, ISO]
- protein kinase binding [ISO]
- receptor binding [ISO]
- tetrahydrobiopterin binding [IDA]
Gene Ontology Cellular Component
ACTN4
Gene Ontology Biological Process
- actin filament bundle assembly [IDA]
- negative regulation of cellular component movement [IDA]
- positive regulation of cellular component movement [IDA, IMP, ISO]
- positive regulation of pinocytosis [IMP]
- protein localization to tight junction [IMP]
- regulation of apoptotic process [ISO]
- tight junction assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Z disc [IDA]
- cell-cell junction [IDA]
- cortical cytoskeleton [IDA]
- cytoplasm [IDA, ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- neuron projection [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- protein complex [ISO]
- pseudopodium [IDA]
- ribonucleoprotein complex [ISO]
- stress fiber [IDA, ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Hypoxia inactivates inducible nitric oxide synthase in mouse macrophages by disrupting its interaction with alpha-actinin 4.
Nitric oxide, produced in macrophages by the high output isoform inducible NO synthase (iNOS), is associated with cytotoxic effects and modulation of Th1 inflammatory/immune responses. Ischemia and reperfusion lead to generation of high NO levels that contribute to irreversible tissue damage. Ischemia and reperfusion, as well as their in vitro simulation by hypoxia and reoxygenation, induce the expression of iNOS ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID