An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Actinfilin, a brain-specific actin-binding protein in postsynaptic density.

Chen Y, Derin R, Petralia RS, Li M

The dynamic assembly and disassembly of actin-based cytoskeleton is closely linked to the changes in the postsynaptic density in both number and shape, which is thought to be important in forming long-term memory. Thus, regulation of actin filaments may play a critical role in contributing to the formation of long-term memory. Here, we report the cloning of actinfilin, a brain-specific ... [more]

J. Biol. Chem. Aug. 23, 2002; 277(34);30495-501 [Pubmed: 12063253]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

KLHL17

Gene Ontology Biological Process

Gene Ontology Molecular Function

POZ domain binding [ISO]actin filament binding [ISO]protein complex scaffold [ISO]

Gene Ontology Cellular Component