PXN
Gene Ontology Biological Process
- activation of MAPK activity [IDA]
- branching morphogenesis of an epithelial tube [IDA]
- cellular component movement [IMP]
- cytoskeleton organization [IMP]
- focal adhesion assembly [IDA]
- growth hormone receptor signaling pathway [ISO]
- integrin-mediated signaling pathway [IMP, TAS]
- lamellipodium assembly [IDA]
- peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of protein kinase activity [IMP]
- regulation of cell shape [IMP]
- substrate adhesion-dependent cell spreading [IMP]
- transforming growth factor beta receptor signaling pathway [IDA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
LIMK1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
LIM kinase 1, a key regulator of actin dynamics, is widely expressed in embryonic and adult tissues.
The expression of endogenous LIM kinase 1 (LIMK1) protein was investigated in embryonic and adult mice using a rat monoclonal antibody (mAb), which recognizes specifically the PDZ domain of LIMK1 and not LIMK2. Immunoblotting analysis revealed widespread expression of LIMK1 existing as a 70-kDa protein in tissues and in cell lines, with a higher mass form (approximately 75 kDa) present ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| LIMK1 PXN | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID