BAIT

ATP6V1G2

1500002D01Rik, Atp6g2, NG38, VAG2, RP23-115O3.3
ATPase, H+ transporting, lysosomal V1 subunit G2
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Differential localization of the vacuolar H+ pump with G subunit isoforms (G1 and G2) in mouse neurons.

Murata Y, Sun-Wada GH, Yoshimizu T, Yamamoto A, Wada Y, Futai M

Vacuolar H(+)-ATPases (V-ATPases), a family of multimeric proton pumps, are involved in a wide variety of physiological processes. We have identified two mouse genes, Atp6g1 and Atp6g2, encoding the G1 and G2 isoforms of the V-ATPase G subunit, respectively. G1 was distributed ubiquitously in the tissues examined, whereas G2 was specifically distributed in central nervous system neurons. G1 was expressed ... [more]

J. Biol. Chem. Sep. 27, 2002; 277(39);36296-303 [Pubmed: 12133826]

Throughput

  • Low Throughput

Curated By

  • BioGRID