BAIT

CHL4

CTF17, MCM17, L000000321, YDR254W
Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; forms a stable complex with Iml3p; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, and Mif2p; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15
Saccharomyces cerevisiae (S288c)
PREY

SCC4

YER147C
Subunit of cohesin loading factor (Scc2p-Scc4p); complex is required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Cohesin-Dependent Association of Scc2/4 with the Centromere Initiates Pericentromeric Cohesion Establishment.

Fernius J, Nerusheva OO, Galander S, Alves FD, Rappsilber J, Marston AL

Cohesin is a conserved ring-shaped multiprotein complex that participates in chromosome segregation, DNA repair, and transcriptional regulation [1, 2]. Cohesin loading onto chromosomes universally requires the Scc2/4 "loader" complex (also called NippedBL/Mau2), mutations in which cause the developmental disorder Cornelia de Lange syndrome in humans [1-9]. Cohesin is most concentrated in the pericentromere, the region surrounding the centromere [10-15]. Enriched ... [more]

Curr. Biol. Mar. 12, 2013; 0(0); [Pubmed: 23499533]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chromosome segregation (APO:0000208)

Additional Notes

  • Scc4-LacI produced in a strain carrying CEN4-proximal lacO repeats, such that Scc4 is tethered to the centromere; chl4 cohesion defect suppressed

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CHL4 SCC4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.4622BioGRID
2035339
CHL4 SCC4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.8821BioGRID
2429283

Curated By

  • BioGRID