TDG
Gene Ontology Biological Process
- DNA demethylation [IDA, IMP]
- base-excision repair [IDA, ISO]
- embryo development [IMP]
- mismatch repair [ISO]
- negative regulation of chromatin binding [IDA]
- negative regulation of protein binding [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IGI, IMP, ISO]
- regulation of gene expression, epigenetic [IMP]
- transcription from RNA polymerase II promoter [IGI]
Gene Ontology Molecular Function- DNA N-glycosylase activity [IDA, ISO]
- RNA polymerase II transcription cofactor activity [IGI]
- SUMO binding [IPI]
- double-stranded DNA binding [ISO]
- mismatched DNA binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [IPI]
- pyrimidine-specific mismatch base pair DNA N-glycosylase activity [ISO]
- structure-specific DNA binding [IDA]
- transcription factor binding [ISO]
- DNA N-glycosylase activity [IDA, ISO]
- RNA polymerase II transcription cofactor activity [IGI]
- SUMO binding [IPI]
- double-stranded DNA binding [ISO]
- mismatched DNA binding [ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein homodimerization activity [ISO]
- protein kinase C binding [IPI]
- pyrimidine-specific mismatch base pair DNA N-glycosylase activity [ISO]
- structure-specific DNA binding [IDA]
- transcription factor binding [ISO]
Gene Ontology Cellular Component
- PML body [IDA]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
GADD45A
Gene Ontology Biological Process
- activation of MAPKKK activity [ISO]
- cellular response to ionizing radiation [ISO]
- centrosome cycle [IGI]
- negative regulation of protein kinase activity [IDA]
- positive regulation of JNK cascade [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of p38MAPK cascade [ISO]
- positive regulation of reactive oxygen species metabolic process [ISO]
- regulation of cell cycle [IDA]
- signal transduction in response to DNA damage [ISO]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Thymine DNA glycosylase is essential for active DNA demethylation by linked deamination-base excision repair.
DNA methylation is a major epigenetic mechanism for gene silencing. Whereas methyltransferases mediate cytosine methylation, it is less clear how unmethylated regions in mammalian genomes are protected from de novo methylation and whether an active demethylating activity is involved. Here, we show that either knockout or catalytic inactivation of the DNA repair enzyme thymine DNA glycosylase (TDG) leads to embryonic ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GADD45A TDG | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID