A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis.

Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Ben Hayun L, Gruetter C, Rauh D, Ori N, Sessa G

Arabidopsis thaliana Brassinosteroid Signaling Kinases (BSKs) constitute a receptor-like cytoplasmic kinase subfamily (RLCK-XII) of 12 members. Previous analysis demonstrated a positive role for BSK1 and BSK3 in the initial steps of brassinosteroid (BR) signal transduction. To investigate the function of BSKs in plant growth and BR signaling, we characterized T-DNA insertion lines within 8 BSK genes (BSK1 to BSK8) and ... [more]

Plant J. Mar. 15, 2013; 0(0); [Pubmed: 23496207]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT5G59010 BSK1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Sreeramulu S (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

BSK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

kinase activity [ISS]protein binding [IPI]

Gene Ontology Cellular Component

AT5G59010