BAIT

TOA2

TFIIA subunit gamma, L000002317, YKL058W
TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; required, along with Toa1p, for ribosomal protein gene transcription in vivo; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

DBP5

RAT8, ATP-dependent RNA helicase DBP5, L000003292, YOR046C
Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus, remodeling messenger ribonucleoprotein particles (mRNPs), with ATPase activity stimulated by Gle1p, IP6 and Nup159p; involved in translation termination along with Sup45p (eRF1); role in the cellular response to heat stress
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Insights into mRNP biogenesis provided by new genetic interactions among export and transcription factors.

Estruch F, Hodge C, Gomez-Navarro N, Peiro-Chova L, Heath CV, Cole CN

The various steps of mRNP biogenesis (transcription, processing and export) are interconnected. It has been shown that the transcription machinery plays a pivotal role in mRNP assembly, since several mRNA export factors are recruited during transcription and physically interact with components of the transcription machinery. Although the shuttling DEAD-box protein Dbp5p is concentrated on the cytoplasmic fibrils of the NPC, ... [more]

BMC Genet. Sep. 12, 2012; 13(0);80 [Pubmed: 22963203]

Throughput

  • Low Throughput

Ontology Terms

  • vegetative growth (APO:0000106)

Additional Notes

  • Both rat8-2 toa2 and rat8-2 toa1 double mutants grew notably more slowly at semi-restrictive temperature than the single mutants

Curated By

  • BioGRID