ATP1A1
Gene Ontology Biological Process
- ATP catabolic process [ISO, TAS]
- cardiac muscle contraction [TAS]
- cellular potassium ion homeostasis [ISO]
- cellular response to mechanical stimulus [IDA]
- cellular response to steroid hormone stimulus [ISO]
- cellular sodium ion homeostasis [ISO]
- dephosphorylation [ISO]
- energy coupled proton transmembrane transport, against electrochemical gradient [TAS]
- membrane hyperpolarization [IMP]
- membrane repolarization [ISO]
- negative regulation of glucocorticoid biosynthetic process [ISO]
- negative regulation of heart contraction [ISO]
- positive regulation of heart contraction [ISO]
- positive regulation of striated muscle contraction [ISO]
- potassium ion import [IDA, ISO]
- potassium ion transmembrane transport [ISO]
- potassium ion transport [IDA]
- regulation of blood pressure [ISO]
- regulation of cardiac muscle cell contraction [IMP]
- regulation of sodium ion transport [IDA]
- regulation of the force of heart contraction [ISO]
- relaxation of cardiac muscle [TAS]
- response to drug [ISO]
- response to glycoside [ISO]
- sodium ion export from cell [ISO]
- sodium ion transmembrane transport [IDA, ISO]
- sodium ion transport [IDA]
Gene Ontology Molecular Function- ADP binding [IDA]
- ATP binding [IDA]
- ankyrin binding [IPI]
- chaperone binding [IDA, ISO]
- phosphatase activity [ISO]
- phosphatidylinositol 3-kinase binding [IPI]
- potassium ion binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- sodium ion binding [IDA]
- sodium:potassium-exchanging ATPase activity [IDA, ISO]
- steroid hormone binding [ISO]
- ADP binding [IDA]
- ATP binding [IDA]
- ankyrin binding [IPI]
- chaperone binding [IDA, ISO]
- phosphatase activity [ISO]
- phosphatidylinositol 3-kinase binding [IPI]
- potassium ion binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- sodium ion binding [IDA]
- sodium:potassium-exchanging ATPase activity [IDA, ISO]
- steroid hormone binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- T-tubule [IDA, ISO]
- apical plasma membrane [ISO]
- basolateral plasma membrane [ISO]
- caveola [IDA]
- endoplasmic reticulum [IDA]
- endosome [IDA]
- extracellular vesicular exosome [ISO]
- integral component of membrane [ISS]
- intercalated disc [IDA]
- membrane [IDA, ISO]
- membrane raft [IDA]
- myelin sheath [ISO]
- neuronal postsynaptic density [ISO]
- plasma membrane [IDA, ISO]
- protein complex [ISO]
- sarcolemma [IDA, ISO]
- sodium:potassium-exchanging ATPase complex [IDA, ISO]
- vesicle [ISO]
ANK3
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [ISO]
- axon guidance [ISO]
- axonogenesis [ISO]
- establishment of protein localization [ISO]
- maintenance of protein location in plasma membrane [ISO]
- membrane assembly [IGI, ISO]
- mitotic cytokinesis [ISO]
- neuromuscular junction development [IEP]
- neuronal action potential [ISO]
- plasma membrane organization [ISO]
- positive regulation of cation channel activity [IMP]
- positive regulation of cell communication by electrical coupling [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of homotypic cell-cell adhesion [IMP]
- positive regulation of membrane depolarization during cardiac muscle cell action potential [IMP]
- positive regulation of membrane potential [IMP]
- positive regulation of protein targeting to membrane [IMP]
- positive regulation of sodium ion transmembrane transporter activity [IMP]
- positive regulation of sodium ion transport [IMP]
- protein localization to plasma membrane [IGI, IMP, ISO]
- protein targeting to plasma membrane [ISO]
- regulation of potassium ion transport [IDA]
- synapse organization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- T-tubule [IDA]
- Z disc [IDA]
- axon [ISO]
- axon initial segment [IDA, ISO]
- basal plasma membrane [ISO]
- basolateral plasma membrane [ISO]
- cell [ISO]
- cell surface [IDA]
- costamere [TAS]
- dendrite [IDA]
- intercalated disc [IDA, ISO]
- intracellular [ISO]
- lateral plasma membrane [ISO]
- membrane [ISO]
- neuromuscular junction [IDA]
- neuron projection [IDA]
- node of Ranvier [IDA, ISO]
- paranode region of axon [ISO]
- plasma membrane [IDA]
- postsynaptic membrane [IDA]
- sarcolemma [IDA, ISO]
- sarcoplasmic reticulum [IDA]
- spectrin-associated cytoskeleton [IDA]
- synapse [ISO]
- tight junction [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Plasma membrane-cytoskeleton-endoplasmic reticulum complexes in neurons and astrocytes.
The possibility that certain integral plasma membrane (PM) proteins involved in Ca(2+) homeostasis form junctional units with adjacent endoplasmic reticulum (ER) in neurons and glia was explored using immunoprecipitation and immunocytochemistry. Rat brain membranes were solubilized with the mild, non-ionic detergent, IGEPAL CA-630. Na(+)/Ca(2+) exchanger type 1 (NCX1), a key PM Ca(2+) transporter, was immunoprecipitated from the detergent-soluble fraction. Several ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID