BAIT

ATP1A2

RATATPA2
ATPase, Na+/K+ transporting, alpha 2 polypeptide
GO Process (33)
GO Function (6)
GO Component (15)
Rattus norvegicus
PREY

ITPR1

I145TR, IP3R1, InsP3R, InsP3R1, P400
inositol 1,4,5-trisphosphate receptor, type 1
GO Process (15)
GO Function (8)
GO Component (24)
Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Plasma membrane-cytoskeleton-endoplasmic reticulum complexes in neurons and astrocytes.

Lencesova L, O'Neill A, Resneck WG, Bloch RJ, Blaustein MP

The possibility that certain integral plasma membrane (PM) proteins involved in Ca(2+) homeostasis form junctional units with adjacent endoplasmic reticulum (ER) in neurons and glia was explored using immunoprecipitation and immunocytochemistry. Rat brain membranes were solubilized with the mild, non-ionic detergent, IGEPAL CA-630. Na(+)/Ca(2+) exchanger type 1 (NCX1), a key PM Ca(2+) transporter, was immunoprecipitated from the detergent-soluble fraction. Several ... [more]

J. Biol. Chem. Jan. 23, 2004; 279(4);2885-93 [Pubmed: 14593108]

Throughput

  • Low Throughput

Curated By

  • BioGRID