BAIT

GUP1

L000004739, YGL084C
Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; role in misfolded protein quality control; proposed to be involved in glycerol transport; GUP1 has a paralog, GUP2, that arose from the whole genome duplication
GO Process (3)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

CWH43

YCR017C
Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion
GO Process (2)
GO Function (0)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Determination and physiological roles of the glycosylphosphatidylinositol lipid remodelling pathway in yeast.

Yoko-O T, Ichikawa D, Miyagishi Y, Kato A, Umemura M, Takase K, Ra M, Ikeda K, Taguchi R, Jigami Y

In the yeast Saccharomyces cerevisiae, glycosylphosphatidylinositol (GPI)-anchored proteins play important roles in cell wall biogenesis/assembly and the formation of lipid microdomains. The lipid moieties of mature GPI-anchored proteins in yeast typically contain either ceramide moieties or diacylglycerol. Recent studies have identified that the GPI phospholipase A2 Per1p and O-acyltransferase Gup1p play essential roles in diacylglycerol-type lipid remodelling of GPI-anchored proteins, ... [more]

Mol. Microbiol. Apr. 01, 2013; 88(1);140-55 [Pubmed: 23421703]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)
  • phenotype: temperature sensitive growth (APO:0000092)
  • phenotype: utilization of nitrogen source (APO:0000099)

Additional Notes

  • cells
  • cwh43D double-mutant cells had higher sensitivity to high
  • double-mutant cells was more severely affected by a low
  • temperature (35oC), calcofluor white and SDS, a detergent
  • than gup1D or cwh43D single-mutant cells
  • that disrupts the cell wall and/or plasma membrane,
  • the growth of gup1D cwh43D, per1D cwh43D
  • the gup1D
  • tryptophan concentration than that of single mutant

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GUP1 CWH43
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-BioGRID
208645
CWH43 GUP1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-6.0142BioGRID
207548
CWH43 GUP1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-15.0097BioGRID
898008

Curated By

  • BioGRID