SMT3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RTF1
Gene Ontology Biological Process
- DNA-templated transcription, termination [IMP]
- global genome nucleotide-excision repair [IMP]
- mRNA 3'-end processing [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- positive regulation of transcription elongation from RNA polymerase I promoter [IDA]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [IMP]
- regulation of chromatin silencing at telomere [IMP]
- regulation of histone H2B conserved C-terminal lysine ubiquitination [IDA, IMP]
- regulation of histone H2B ubiquitination [IMP]
- regulation of histone H3-K4 methylation [IMP]
- regulation of histone H3-K79 methylation [IMP]
- regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- regulation of transcription from RNA polymerase II promoter [IGI]
- regulation of transcription-coupled nucleotide-excision repair [IGI]
- snoRNA 3'-end processing [IMP]
- snoRNA transcription from an RNA polymerase II promoter [IMP]
- transcription elongation from RNA polymerase II promoter [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
Global analysis of SUMO chain function reveals multiple roles in chromatin regulation.
Like ubiquitin, the small ubiquitin-related modifier (SUMO) proteins can form oligomeric "chains," but the biological functions of these superstructures are not well understood. Here, we created mutant yeast strains unable to synthesize SUMO chains (smt3(allR)) and subjected them to high-content microscopic screening, synthetic genetic array (SGA) analysis, and high-density transcript profiling to perform the first global analysis of SUMO chain ... [more]
Throughput
- High Throughput
Ontology Terms
- colony size (APO:0000063)
Additional Notes
- SGA analysis; decreased colony size; smt3allR allele, in which all nine lysine codons have been mutated to code for arginine; the resulting polypeptide can be conjugated to proteins as a monomer, but does not form SUMO chains.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SMT3 RTF1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1682 | BioGRID | 1973996 |
Curated By
- BioGRID