Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes.

Nebl T, Pestonjamasp KN, Leszyk JD, Crowley JL, Oh SW, Luna EJ

Plasma membranes are organized into functional domains both by liquid-ordered packing into "lipid rafts," structures that resist Triton extraction, and by attachments to underlying cytoskeletal proteins in assemblies called "membrane skeletons." Although the actin cytoskeleton is implicated in many lipid raft-mediated signaling processes, little is known about the biochemical basis for actin involvement. We show here that a subset of ... [more]

J. Biol. Chem. Nov. 08, 2002; 277(45);43399-409 [Pubmed: 12202484]

Throughput

  • Low Throughput

Curated By

  • BioGRID